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Intermediate

Part:BBa_M31500:Design

Designed by: Tiffany Guo   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-27)


refactored M13K07 phage fragment


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 225
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 225
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 225
    Illegal BamHI site found at 3399
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 225
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 225
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Had to make sure 1) overlapping promoter/rbs/orf sequences were not repeated, 2) restriction enzyme sites were unique and did not cut through important parts.


Source

M13K07 fragment from bp 1-2222 (HpaI site to BamHI site)

References